Site na Jenụwarị 2020 ruo Ọktọba 2020, a na-eme usoro ọmụmụ ihe na ụlọ ọgwụ Mahadum Pisa nke bipụtara na BMC Microbiology.Goldstream®A na-eji Fungus (1-3) -β-D-Glucan Test iji chọpụta ọkwa BDG site na nlele BAL.A gbakọtara nsonaazụ ya site na aIhe na-agụ Kinetic Tube zuru oke IGL-200sitere na Era Biology.Nchoputa a gosiputara na BDG kwesiri iburu n'uche maka uru amuma di elu di elu.Ọ rụpụtara uru na-ewepụ nchoputa nke PCP maka ndị ọrịa niile na-adịghị mma.
ndabere:
Ọkọlọtọ ọla edo diagnostic dị ugbu a makaỌrịa pneumocystisA na-anọchi anya ya site na nleba anya nke ero site na nlele iku ume ụlọ ọgwụ, dị ka mmiri bronchoalveolar-lavage, na-akọwa "pụtara"P. jiroveciioyi oyi, ebe qPCR na-enye ohere ịkọwapụta nchoputa "nwere ike ime", n'ihi na ọ nweghị ike ịkpa ókè ọrịa sitere na nchịkwa.Otú ọ dị, ụzọ molekụla, dị ka njedebe PCR na qPCR, na-adị ngwa ngwa, dị mfe ịrụ ọrụ na ịtụgharị uche, si otú a na-enye ohere ka ụlọ nyocha ahụ nyeghachi onye dọkịta ahụ data microbiological bara uru n'ime obere oge.Ọmụmụ ihe dị ugbu a bụ iji atụnyere microscopy na nyocha mkpụrụ ndụ yana arụmọrụ beta-D-glucan diagnostic na mmiri bronchoalveolar-lavage sitere na ndị ọrịa a na-enyo enyo.Ọrịa pneumocystisọrịa oyi.Mmiri Bronchoalveolar-lavage sitere na ihe ize ndụ iri na asatọ na ihe nchịkwa anọ na-adịghị mma mere Grocott-Gomori's methenamine silver-staining, PCR end-point, RT-PCR, na beta-D-glucan assay.
Nsonaazụ:
All microscopically nti bronchoalveolar-lavage samples (50%) pụtakwara nti site na njedebe na ezigbo oge PCR na ihe niile, ma abụọ, pụtakwara mma site na beta-D-glucan quantification.PCR njedebe na RT-PCR achọpụtala 10 (55%) na 11 (61%) n'ime ihe nlele iri na asatọ ahụ, n'otu n'otu, si otú a na-egosi mmụba dị elu ma e jiri ya tụnyere microscopy.Achọpụtara RT-PCR niile nwere Ct<27 n'ụzọ nyocha, ebe nlele nwere Ct≥ 27 abụghị.
Nkwubi okwu:
Ọrụ anyị na-egosipụta mkpa ọ dị ịmegharị na ịkọwapụta ọrụ nke nchọpụta mkpụrụ ndụ na ọnọdụ ụlọ ọgwụ pụrụ iche, dị ka.P. jiroveciiọrịa, nke bụ ihe a na-adịghị ahụkebe mana ọ dịkwa njọ na ọnọdụ ụlọ ọgwụ na-aga n'ihu ngwa ngwa na-emetụta ndị ọbịa na-enweghị mgbochi nke ga-erite uru na nyocha ngwa ngwa.Ndị ọrịa ahọpụtara nke ọma, dị ka njirisi nsonye si dị, a ga-ewepụ ihe na-adịghị mma site na usoro molekụlaP. jiroveciiọrịa oyi.
Franconi I, Leonildi A, Erra G, et al.Tụnyere usoro microbiological dị iche iche maka nyocha nke Pneumocystis jirovecii ka oyi baa na mmiri bronchoalveolar-lavage.BMC Microbiol.2022;22 (1):143 .Ebipụtara 2022 Mee 21. doi:10.1186/s12866-022-02559-1
Oge nzipu: Jun-14-2022