Usoro usoro ndị a bụ nyocha site na iji antigen a kapịrị ọnụ iji chọpụta ọgwụ mgbochi ọrịa na serum ndị ọrịa, gụnyere nchọpụta ọgwụ mgbochi IgM na nha mgbochi IgG.Ọgwụ mgbochi IgM na-apụ n'anya n'ime ọtụtụ izu, ebe ọgwụ mgbochi IgG na-adịgide ruo ọtụtụ afọ.A na-emezu nyocha nke ọrịa nje site na serologically site n'igosi ịrị elu nke antibody titer na nje ma ọ bụ site n'igosipụta ọgwụ mgbochi nje nke klas IgM.Ụzọ ndị e ji mee ihe gụnyere nnwale neutralization (Nt), ule mmeju (CF), ule hemagglutination inhibition (HI), na nyocha immunofluorescence (IF), hemagglutination passive, na immunodiffusion.
A. Nnwale Nnọpụiche
N'oge ọrịa na-efe efe ma ọ bụ omenala cell, nje nwere ike igbochi ya kpọmkwem mgbochi nje ma na-efunahụ ọrịa, a na-akọwa ụdị mgbochi a dị ka neutralization antibody.Nnwale a na-anọpụ iche bụ ịchọpụta ihe mgbochi mgbochi na serum ndị ọrịa.
B. Nleba anya mmezi mmezi
Enwere ike iji nyocha mmezi mmezi ahụ iji chọpụta ọnụnọ nke antibody ma ọ bụ antigen akọwapụtara na serum onye ọrịa.Nnwale ahụ na-eji mkpụrụ ndụ ọbara uhie uhie (SRBC), mgbochi SRBC mgbochi na mmeju, yana antigen (ọ bụrụ na-achọ mgbochi na serum) ma ọ bụ mgbochi kpọmkwem (ọ bụrụ na-achọ antigen na serum).
C. Nnyocha Mgbochi Hemagglutination
Ọ bụrụ na ọnụọgụ nje dị na nlele dị elu, mgbe agwakọtara ihe nlele ahụ na RBC, a ga-emepụta lattice nke nje na RBC.A na-akpọ ihe omume a hemagglutination.Ọ bụrụ na ọgwụ mgbochi megide hemagglutinins dị, a ga-egbochi hemaglutination.N'oge ule mgbochi hemagglutination, a na-agwakọta dilutions nke serum na nje amaara ama.Mgbe incubation gasịrị, a na-agbakwunye RBC, a na-ahapụkwa ngwakọta ahụ ka ọ nọrọ ọdụ ruo ọtụtụ awa.Ọ bụrụ na egbochiri hemagglutination, pellet nke RBC na-etolite na ala nke tube.Ọ bụrụ na egbochighị hemaglutination, a na-emepụta ihe nkiri dị mkpa.
Oge nzipu: Ọktoba 15-2020