Nchọpụta NDM nke Carbapenem na-eguzogide K-Set (Nleba Nleba Ọdịnihu)
Okwu Mmalite ngwaahịa
Nchọpụta NDM nke Carbapenem na-eguzogide K-Set (Lateral Flow Assay) bụ usoro nyocha nke immunochromatographic ezubere maka nchọpụta qualitative nke ụdị carbapenemase NDM na mpaghara nje.Nnwale ahụ bụ nyocha ụlọ nyocha nke nwere ike inye aka n'ịchọpụta ụdị ụdị carbapenem na-eguzogide NDM.
Njirimara
| Aha | Nchọpụta NDM nke Carbapenem na-eguzogide K-Set (Nleba Nleba Ọdịnihu) |
| Usoro | Nleba anya Flow Assay |
| Ụdị ihe atụ | Ogbe nje bacteria |
| Nkọwapụta | 25 ule / ngwa |
| Oge nchọpụta | 10-15 min |
| Ihe nchọpụta | Enterobacteriaceae na-eguzogide Carbapenem (CRE) |
| Ụdị nchọpụta | NDM |
| Nkwụsi ike | K-Set kwụsiri ike maka afọ 2 na 2°C-30°C |
Uru
- Ngwa ngwa
Nweta nsonaazụ n'ime nkeji 15, ụbọchị 3 tupu ụzọ nchọpụta ọdịnala - Dị mfe
Ọ dị mfe iji, ndị ọrụ ụlọ nyocha nkịtị nwere ike ịrụ ọrụ na-enweghị ọzụzụ - ziri ezi
Nnukwu uche na nkọwapụta
Oke nchọpụta dị ala: 0.15 ng/ml
Nwere ike ịchọpụta ọtụtụ n'ime subụdị nkịtị nke NDM
- Nsonaazụ kenuche
Ọ dịghị mkpa maka ngụkọta oge, visual agụ nsonaazụ - Akụ na ụba
Enwere ike ibuga ma chekwaa ngwaahịa na ụlọ okpomọkụ, na-ebelata ọnụ ahịa
Mkpa ule CRE
Enterobacteriaceae na-eguzogide Carbapenem (CRE) bụ ụdị nje bacteria.Ha nwere ike ibute ọrịa siri ike nke nwere ike isi ike ịgwọ.CRE nwetara aha ha site n'eziokwu na ha na-eguzogide ọgwụ carbapenems.Carbapenems bụ klas dị elu nke ọgwụ nje.E kere ha n'afọ ndị 1980 ka ha nyere aka na-agwọ nje bacteria na-enweghị ike iji ọgwụ nje ndị ọzọ gwọọ ha.A na-eji ọgwụ nje gbuo ụfọdụ ụdị nje bacteria.Enwere ọtụtụ ụdị ọgwụ ndị a.Ka oge na-aga, ụfọdụ nje bacteria nwere ike gharakwa igbu ha.A maara nke a dị ka ọgwụ mgbochi.Mgbasa ngwa ngwa nke CRE sitere na iji ọgwụ eme ihe na ijikwa ndị ọrịa CRE na-ekwesịghị ekwesị.Ọ bụrụ na elebaraghị ọnọdụ ahụ anya, ọ ga-emetụta ahụike ahụike mmadụ nke ukwuu, na-eme ka ọgwụgwọ ụlọ ọgwụ na njikwa ọrịa sie ike karị.
Usoro a na-emekarị iji gbochie mgbasa nke CRE bụ:
- Nyochaa ọrịa CRE nke ọma na ụlọ ọgwụ
- Ibelata usoro ọgwụgwọ mmegide ozugbo
- Kewapụ ndị ọrịa CRE
- Depụta ọgwụ nje naanị ma ọ bụrụ na ọ dị mkpa n'ezie
- Iji usoro adịghị mma iji belata mgbasa
……
O doro anya ịhụ mkpa nyocha mbụ nke CRE na ụzọ niile dị n'elu.Nnwale nyocha ngwa ngwa na nke ziri ezi dị oke mkpa maka mbido mbụ nke ụdị CRE, ntụzịaka nke ọgwụ, yana nkwalite usoro ahụike na ahụike mmadụ.
NDM-ụdị carbapenemase
Carbapenemase na-ezo aka n'ụdị β-lactamase nke nwere ike ọ dịkarịa ala hydrolyze imipenem ma ọ bụ meropenem, gụnyere A, B, D ụdị atọ nke enzymes nke Ambler molecular Ọdịdị.N'ime ha, Klas B bụ metallo-β-lactamases (MBL), gụnyere IMP, VIM na NDM, wdg, nke a na-akpọ metalloenzyme, nke a na-achọta na Pseudomonas aeruginosa, Acinetobacteria na Enterobacteriaceae bacteria.Ebe ọ bụ na mbụ a kọrọ na India na 2008, NDM (New Delhi metallo-beta-lactamase) agbasawo n'ụwa niile na ọnụ ọgụgụ dị egwu.Ka ọ dị ugbu a, NDM apụtala n'ọtụtụ mba dị na Europe, United States, Canada na Mexico na North America, yana mba Eshia dịka China, Japan, South Korea na Singapore.Na mba ndị dị ka India na Pakistan, NDM ebutela ọrịa na-efe efe, yana ọnụọgụ nchọpụta nke 38.5%.Ịmepụta ngwa ngwa ngwa ngwa nke carbapenemase bụ ihe dị oke mkpa maka mmalite nke ụdị ụdị ọgwụ na-eguzogide ọgwụ, nduzi nke ọgwụ, yana nkwalite usoro ahụike na ahụike mmadụ.
Ọrụ
- Tinye 5 tụlee nke ngwọta ọgwụgwọ sample
- Jiri loop inoculation tinye n'ime ụlọ ndị nje bacteria
- Fanye akaghị n'ime tube
- Tinye 50 μL na S nke ọma, chere 10-15 nkeji
- Gụọ nsonaazụ ya
Ozi ịtụ
| Nlereanya | Nkọwa | Koodu ngwaahịa |
| CPN-01 | 25 ule / ngwa | CPN-01 |
